Effect of Biosynthesized Silver Nanoparticles on Staphylococcus aureus Biofilm Quenching and Prevention of Biofilm Formation
Corresponding Author: Pratik R. Chaudhari
Nano-Micro Letters,
Vol. 4 No. 1 (2012), Article Number: 34-39
Abstract
The development of green experimental processes for the synthesis of nanoparticles is a need in the field of nanotechnology. The synthesis of silver nanoparticles was achieved using Bacillus cereus supernatant and 1 mM silver nitrate. 100 mM glucose was found to quicken the rate of reaction of silver nanoparticles synthesis. UV-visible spectrophotometric analysis was carried out to assess the synthesis of silver nanoparticles. The synthesized silver nanoparticles were further characterized by using Nanoparticle Tracking Analyzer (NTA), Transmission Electron Microscope and Energy Dispersive X-ray spectra. These silver nanoparticles showed enhanced quorum quenching activity against Staphylococcus aureus biofilm and prevention of biofilm formation which can be seen under inverted microscope (40 X). The synergistic effect of silver nanoparticles along with antibiotics in biofilm quenching was found to be effective. In the near future, silver nanoparticles could be used in the treatment of infections caused by highly antibiotic resistant biofilm.
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- Y. Park, Y. N. Hong, A. Weyers, Y. S. Kim and R. J. Linhardt, IET Nanobiotechnology 5, 69 (2011).
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- N. Saifuddin, C. W. Wong and A. N. Yasumira, E-J. Chem 6, 61 (2009).
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- G. H. Bowden. and I. R. Hamilton, Crit. Rev. Oral. Biol. Med 9, 54 (1998). http://dx.doi.org/10.1177/10454411980090010401
- K. Lewis, Antimicrob. Agents Chemother. 45, 999 (2001). http://dx.doi.org/10.1128/AAC.45.4.999-1007.2001
- J. W. Costerton, P. Stewart and E. P. Greenberg, Science 284, 1318 (1999). http://dx.doi.org/10.1126/science.284.5418.1318
- D. Monroe, PLoS Biology 5, 2458 (2007). http://dx.doi.org/10.1371/journal.pbio.0050307
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- E. O. King and H. W. Tatum, J. Infect. Dis. 111, 85 (1962). http://dx.doi.org/10.1093/infdis/111.2.85
- K. Raja, N. Selvaraj, E. Krishnamoorthi and B. K. Singh, IJPI’s J. Biotechnology Biotherapeutics. 1, 1 (2011).
- S. Akanna, K. V. Prasad, E. K. Elumalai and N. Savithramma, Digest J. Nanomater. Biostruct. 5, 369 (2010).
References
Y. Park, Y. N. Hong, A. Weyers, Y. S. Kim and R. J. Linhardt, IET Nanobiotechnology 5, 69 (2011).
G. Schmid, Chem. Rev. 92, 1709 (1992). http://dx.doi.org/10.1021/cr00016a002
K. Kalimuthu, R. S. Babu, D. Venkataraman, B. Mohd and S. Gurunathan, Colloids Surf. B 65, 150 (2008). http://dx.doi.org/10.1016/j.colsurfb.2008.02.018
P. R. Chaudhari, S. A. Masurkar, V. B. Shidore and S. P. Kamble, Int. J. Pharma and Bio Sciences 3, 222 (2012).
N. Saifuddin, C. W. Wong and A. N. Yasumira, E-J. Chem 6, 61 (2009).
A. Ahmad, P. Mukherjee, S. Senapati, D. Mandal, M. I. Khan and R. Kumar, Colloids Surf. B 28, 313 (2003). http://dx.doi.org/10.1016/S0927-7765(02)00174-1
K. Kathiresan., S. Manivannan, M. A. Nabeel and B. Dhivya, Colloids Surf. B 71, 133 (2009). http://dx.doi.org/10.1016/j.colsurfb.2009.01.016
R. Raut, J. R. Lakkakula, N. Kolekar, V. D. Mendhulkar and S. B. Kashid, Nano-Micro Lett. 2, 106 (2010). http://dx.doi.org/10.5101/nml.v2i2.p106-113
S. A. Masurkar, P. R. Chaudhari, V. B. Shidore and S. P. Kamble, Nano-Micro Lett. 3, 189 (2011). http://dx.doi.org/10.5101/nml.v3i3.p189-194
G. H. Bowden. and I. R. Hamilton, Crit. Rev. Oral. Biol. Med 9, 54 (1998). http://dx.doi.org/10.1177/10454411980090010401
K. Lewis, Antimicrob. Agents Chemother. 45, 999 (2001). http://dx.doi.org/10.1128/AAC.45.4.999-1007.2001
J. W. Costerton, P. Stewart and E. P. Greenberg, Science 284, 1318 (1999). http://dx.doi.org/10.1126/science.284.5418.1318
D. Monroe, PLoS Biology 5, 2458 (2007). http://dx.doi.org/10.1371/journal.pbio.0050307
M. Darroudi, M. B. Ahmad, A. H. Abdlahul, N. A. Ibrahim and K. Shameli, Int. J. Mol. Sci. 11, 3898 (2010). http://dx.doi.org/10.3390/ijms11103898
J. B. Kaplan, C. Ragunath, N. Ramasubbu and H. Daniel, J. Bacteriol. 185, 4693 (2003). http://dx.doi.org/10.1128/JB.185.16.4693-4698.2003
E. O. King and H. W. Tatum, J. Infect. Dis. 111, 85 (1962). http://dx.doi.org/10.1093/infdis/111.2.85
K. Raja, N. Selvaraj, E. Krishnamoorthi and B. K. Singh, IJPI’s J. Biotechnology Biotherapeutics. 1, 1 (2011).
S. Akanna, K. V. Prasad, E. K. Elumalai and N. Savithramma, Digest J. Nanomater. Biostruct. 5, 369 (2010).