Issue

Vol. 3 No. 2 (2011)

Development of an Amperometric Hydrogen Peroxide Biosensor based on the Immobilization of Horseradish Peroxidase onto Nickel Ferrite Nanoparticle-Chitosan Composite

https://doi.org/10.1007/BF03353657
Furkan Yalçıner
Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B. Cekmece, Istanbul 34500, Turkey
Emre Çevik
Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul, 34500, Turkey
Mehmet Şenel
Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul, 34500, Turkey
Abdülhadi Baykal
Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul, 34500, Turkey

Corresponding Author: Mehmet Şenel

msenel@fatih.edu.tr

Nano-Micro Letters, Vol. 3 No. 2 (2011), Article Number: 91-98

Abstract

Nickel ferrite (NiFe2O4) nanoparticles have been dispersed in chitosan solution in order to fabricate nanocomposite films. Horseradish peroxidase (HRP) has been immobilized onto this chitosan-NiFe2O4 nanocomposite film via physical adsorption. The size of the NiFe2O4 nanoparticles has been estimated using X-ray diffraction pattern and scanning electron microscopy (SEM) to be 40±9 nm. The chitosan-NiFe2O4 nanocomposite film and HRP/chitosan-NiFe2O4 bioelectrode have been characterized using SEM technique. The HRP/chitosan-NiFe2O4 nanocomposite bioelectrode has a response time of 4 s, linearity as 0.3 to 12mM of H2O2, sensitivity as 22 nA/mM. The effects of pH and the temperature of the immobilized HRP electrode have also been studied.

Keywords

Biosensor NiFe2O4 Horseradish peroxidase Nanoparticle Chitosan
Yalçıner, Furkan, Emre Çevik, Mehmet Şenel, and Abdülhadi Baykal. 2011. “Development of an Amperometric Hydrogen Peroxide Biosensor Based on the Immobilization of Horseradish Peroxidase onto Nickel Ferrite Nanoparticle-Chitosan Composite”. Nano-Micro Letters 3 (2):91-98. https://doi.org/10.1007/BF03353657.
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