Synthesis, characterization and biocompatibility studies of zinc oxide (ZnO) nanorods for biomedical application

R. Gopikrishnan; K. Zhang; P. Ravichandran; S. Baluchamy; V. Ramesh; S. Biradar; P. Ramesh; J. Pradhan; J. C. Hall; A. K. Pradhan; G. T. Ramesh

doi:10.1007/BF03353614

Issue

Vol. 2 No. 1 (2010)

Issue Published : Jan 1, 2010

Synthesis, characterization and biocompatibility studies of zinc oxide (ZnO) nanorods for biomedical application

https://doi.org/10.1007/BF03353614

R. Gopikrishnan

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

K. Zhang

Center for Materials Research, Norfolk State University, 700 Park Avenue, Norfolk, VA 23504, USA

P. Ravichandran

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

S. Baluchamy

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

V. Ramesh

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

S. Biradar

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

P. Ramesh

Health Science Academy, Bayside High School, Virginia Beach, 4960 Haygood Road, VA 23455, USA

J. Pradhan

Center for Materials Research, Norfolk State University, 700 Park Avenue, Norfolk, VA 23504, USA

J. C. Hall

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

A. K. Pradhan

Center for Materials Research, Norfolk State University, 700 Park Avenue, Norfolk, VA 23504, USA

G. T. Ramesh

Molecular Toxicology Laboratory, Center for Biotechnology and Biomedical Sciences, Norfolk State University, 700 Park Avenue, Norfolk, VA, 23504, USA

Corresponding Author: G. T. Ramesh

gtramesh@nsu.edu

Nano-Micro Letters, Vol. 2 No. 1 (2010), Article Number: 31-36
Article Published : Mar 23, 2010

Abstract

Nanoparticles are increasingly being recognized for their potential utility in biological applications including nanomedicine. Here, we have synthesized zinc oxide (ZnO) nanorods using zinc acetate and hexamethylenetetramine as precursors followed by characterizing using X-ray diffraction, fourier transform infrared spectroscopy, scanning electron microscopy and transmission electron microscopy. The growth of synthesized zinc oxide nanorods was found to be very close to its hexagonal nature, which is confirmed by X-ray diffraction. The nanorod was grown perpendicular to the long-axis and grew along the [001] direction, which is the nature of ZnO growth. The morphology of synthesized ZnO nanorods from the individual crystalline nucleus was confirmed by scanning and transmission electron microscopy. The length of the nanorod was estimated to be around 21 nm in diameter and 50 nm in length. Our toxicology studies showed that synthesized ZnO nanorods exposure on hela cells has no significant induction of oxidative stress or cell death even in higher concentration (10 μg/ml). The results suggest that ZnO nanorods might be a safer nanomaterial for biological applications.

Keywords

Zinc oxide [ZnO] Nanorods XRD SEM & TEM Cytotoxicity

Gopikrishnan, R., K. Zhang, P. Ravichandran, S. Baluchamy, V. Ramesh, S. Biradar, P. Ramesh, J. Pradhan, J. C. Hall, A. K. Pradhan, and G. T. Ramesh. 2010. “Synthesis, Characterization and Biocompatibility Studies of Zinc Oxide (ZnO) Nanorods for Biomedical Application”. Nano-Micro Letters 2 (1):31-36. https://doi.org/10.1007/BF03353614.

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A. Nel, T. Xia, L. Madler and N. Li, Science 311, 622 (2006). doi:10.1126/science.1114397

D. A. Groneberg, M. Giersig, T. Welte and U. Pison, Curr. Drug Targets. 7, 643 (2006). doi:10.2174/1389450067 77435245

D. M. Balshaw, M. Philbert and W. A. Suk, Toxicol. Sci. 88, 298 (2005). doi:10.1093/toxsci/kfi312

Y. Lee, D. S. Ruby, D. W. Peters, B. B. McKenzie and J. P. Hsu, Nano Lett. 8, 1501 (2008). doi:10.1021/nl080659j

T. Thomas, K. Thomas, N. Sadrieh, N. Savage, P. Adair and R. Bronaugh, Toxicol. Sci. 91, 14 (2006). doi:10.1093/ toxsci/kfj129

T. Ma, M. Guo, M. Zhang, Y. Zhang and X. Wang, Nanotech. 18, 035605 (2007). doi:10.1088/0957-4484/ 18/3/035605

U. O. Zgur, Y. I, Alivov, C. Liu, A. Teke, M. A. Reshchikov, S. Dogan, V. Avrutin, S. J. Cho and H. Morkoc, J. Appl. Phys. 98, 041301 (2005).

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J. G. Strom, H. W. Jun and J. Pharm. Sci. 69, 1261 (1980). doi:10.1002/jps.2600691107

S. Sarkar, C. Sharma, R. Yog, A. Periakaruppan, O. Jejelowo, R. Thomas, E. V. Barrer, A. C. Rice-Ficht, B. L. Wilson, G. T. Ramesh and J. Nanosci, Nanotechnology 7, 584 (2007).

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J. Tang and X. Yang, Mat. Lett. 60, 3487 (2006). doi:10.1016/j.matlet.2006.03.037

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J. H. Yuan, Y. Chen, H. X. Zha, L. J. Song, C. Y. Li, J. Q. Li and X. H. Xia, Colloids and Surfaces B: Biointerfaces 76, 145 (2010). doi:10.1016/j.colsurfb.2009.10.028

K. M. Reddy, K. Feris, J. Bell, D. J. Wingett, C. Hanley and A. Punnoose, Appl. Phys. Lett. 90, 213902 (2007). doi:10.1063/1.2742324

H. Sies, Free. Radic. Biol. Med. 27, 916 (1999). doi:10.10 16/S0891-5849(99)00177-X