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Vol. 10 No. 2 (2018)

Hierarchical Nanogold Labels to Improve the Sensitivity of Lateral Flow Immunoassay

https://doi.org/10.1007/s40820-017-0180-2
Kseniya Serebrennikova
Chemistry Faculty, Lomonosov Moscow State University, Leninskiye Gory, Moscow, Russia 119991
Jeanne Samsonova
Chemistry Faculty, Lomonosov Moscow State University, Leninskiye Gory, Moscow, Russia 119991
Alexander Osipov
Chemistry Faculty, Lomonosov Moscow State University, Leninskiye Gory, Moscow, Russia 119991

Corresponding Author: Kseniya Serebrennikova

ksenijasereb@mail.ru

Nano-Micro Letters, Vol. 10 No. 2 (2018), Article Number: 24

Abstract

Lateral flow immunoassay (LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation. However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications. In this study, spherical nanogold labels along with new types of nanogold labels such as gold nanopopcorns and nanostars were prepared, characterized, and applied for LFIA of model protein antigen procalcitonin. It was found that the label with a structure close to spherical provided more uniform distribution of specific antibodies on its surface, indicative of its suitability for this type of analysis. LFIA using gold nanopopcorns as a label allowed procalcitonin detection over a linear range of 0.5–10 ng mL−1 with the limit of detection of 0.1 ng mL−1, which was fivefold higher than the sensitivity of the assay with gold nanospheres. Another approach to improve the sensitivity of the assay included the silver enhancement method, which was used to compare the amplification of LFIA for procalcitonin detection. The sensitivity of procalcitonin determination by this method was 10 times better the sensitivity of the conventional LFIA with gold nanosphere as a label. The proposed approach of LFIA based on gold nanopopcorns improved the detection sensitivity without additional steps and prevented the increased consumption of specific reagents (antibodies).


Highlights:


1 New types of nanogold labels were evaluated for their improved sensitivity in procalcitonin lateral flow immunoassay (LFIA).
2 Gold nanostars and nanopopcorns were applied as a label in a sandwich-format LFIA.
3 The use of gold nanopopcorns as a label demonstrated a fivefold increase in sensitivity compared to that associated with the conventional LFIA based on 20-nm gold nanospheres.

Keywords

Lateral flow immunoassay Gold nanosphere Gold nanopopcorn Gold nanostar Silver enhancement Procalcitonin
Serebrennikova, Kseniya, Jeanne Samsonova, and Alexander Osipov. 2017. “Hierarchical Nanogold Labels to Improve the Sensitivity of Lateral Flow Immunoassay”. Nano-Micro Letters 10 (2):24. https://doi.org/10.1007/s40820-017-0180-2.
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References
  1. S. Wang, Y. Quan, N. Lee, I.R. Kennedy, Rapid determination of fumonisin B1 in food samples by enzyme-linked immunosorbent assay and colloidal gold immunoassay. J. Agric. Food Chem. 54(7), 2491–2495 (2006). https://doi.org/10.1021/jf0530401
  2. J. Zhu, N. Zou, D. Zhu, J. Wang, Q. Jin, J. Zhao, H. Mao, Simultaneous detection of high-sensitivity cardiac troponin I and myoglobin by modified sandwich lateral flow immunoassay: proof of principle. Clin. Chem. 57(12), 1732–1738 (2011). https://doi.org/10.1373/clinchem.2011.171694
  3. Y.-Y. Lin, J. Wang, G. Liu, H. Wu, C.M. Wai, Y. Lin, A Nanop label/immunochromatographic electrochemical biosensor for rapid and sensitive detection of prostate-specific antigen. Biosens. Bioelectron. 23(11), 1659–1665 (2008). https://doi.org/10.1016/j.bios.2008.01.037
  4. C. Chen, J. Wu, A fast and sensitive quantitative lateral flow immunoassay for cry1Ab based on novel signal amplification conjugate. Sensors 12(9), 11684–11696 (2012). https://doi.org/10.3390/s120911684
  5. N.V. Zaytseva, R.A. Montagna, E.M. Lee, A.J. Baeumner, Multi-analyte single-membrane biosensor for the serotype-specific detection of dengue virus. Anal. Bioanal. Chem. 380(1), 46–53 (2004). https://doi.org/10.1007/s00216-004-2724-9
  6. S.J. Yeo, D.T. Huong, N.N. Hong, C.-Y. Li, K. Choi et al., Rapid and quantitative detection of zoonotic influenza a virus infection utilizing coumarin-derived dendrimer-based fluorescent immunochromatographic strip test (FICT). Theranostics 4(12), 1239–1249 (2014). https://doi.org/10.7150/thno.10255
  7. D. Tang, J.C. Sauceda, Z. Lin, S. Ott, E. Basova et al., Magnetic nanogold microspheres-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food. Biosens. Bioelectron. 25(2), 514–518 (2009). https://doi.org/10.1016/j.bios.2009.07.030
  8. K. Zhang, J. Wu, Y. Li, Y. Wu, T. Huang, D. Tang, Hollow nanogold microsphere-signalized lateral flow immunodipstick for the sensitive determination of the neurotoxin brevetoxin B. Microchim. Acta 181(11), 1447–1454 (2014). https://doi.org/10.1007/s00604-014-1291-9
  9. Z. Gao, H. Ye, D. Tang, J. Tao, S. Habibi, A. Minerick, D. Tang, X. Xia, Platinum-decorated gold nanops with dual functionalities for ultrasensitive colorimetric in vitro diagnostics. Nano Lett. 17(9), 5572–5579 (2017). https://doi.org/10.1021/acs.nanolett.7b02385
  10. J. Chandler, T. Gurmin, N. Robinson, The place of gold in rapid tests. IVD Technol. 6(2), 37–49 (2000)
  11. J. Aveyard, M. Mehrabi, A. Cossins, H. Braven, R. Wilson, One step visual detection of PCR products with gold nanops and a nucleic acid lateral flow (NALF) device. Chem. Commun. 41(41), 4251–4253 (2007). https://doi.org/10.1039/b708859k
  12. I. Safenkova, A. Zherdev, B. Dzantiev, Factors influencing the detection limit of the lateral-flow sandwich immunoassay: a case study with potato virus X. Anal. Bioanal. Chem. 403(6), 1595–1605 (2012). https://doi.org/10.1007/s00216-012-5985-8
  13. B. Khlebtsov, N. Khlebtsov, Enhanced solid-phase immunoassay using gold nanoshells: effect of nanop optical properties. Nanotechnology 19(43), 435703 (2008). https://doi.org/10.1088/0957-4484/19/43/435703
  14. K. Omidfar, S. Kia, S. Kashanian, M. Paknejad, A. Besharatie, S. Kashanian, B. Larijani, Colloidal nanogold-based immunochromatographic strip test for the detection of digoxin toxicity. Appl. Biochem. Biotechnol. 160(3), 843–855 (2010). https://doi.org/10.1007/s12010-009-8535-x
  15. L. Dykman, N. Khlebtsov, Gold nanops in biology and medicine: Recent advances and prospects. Acta Nat. 3(2), 34–55 (2011). http://pubmedcentralcanada.ca/pmcc/s/PMC3347577/
  16. L.-X. Chen, J.-J. Lv, A.-J. Wang, H. Huang, J.-J. Feng, One-step wet-chemical synthesis of gold nanoflower chains as highly active surface-enhanced Raman scattering substrates. Sens. Actuators B 222, 937–944 (2016). https://doi.org/10.1016/j.snb.2015.09.010
  17. P. Truong, B. Kima, S. Sim, Rational aspect ratio and suitable antibody coverage of gold nanorod for ultra-sensitive detection of a cancer biomarker. Lab Chip 12(6), 1102–1109 (2012). https://doi.org/10.1039/c2lc20588b
  18. J. Li, J. Wu, X. Zhang, Y. Liu, D. Zhou, H. Sun, H. Zhang, B. Yang, Controllable synthesis of stable urchin-like gold nanops using hydroquinone to tune the reactivity of gold chloride. J. Phys. Chem. C 115(9), 3630–3637 (2011). https://doi.org/10.1021/jp1119074
  19. E. Hao, R. Bailey, G. Schatz, J. Hupp, S. Li, Synthesis and optical properties of branched gold nanocrystals. Nano Lett. 4(2), 327–330 (2004). https://doi.org/10.1021/nl0351542
  20. H. Yuan, C.G. Khoury, H. Hwang, C.M. Wilson, G.A. Grant, T. Vo-Dinh, Gold nanostars: surfactant-free synthesis, 3D modelling, and two-photon photoluminescence imaging. Nanotechnology 23(7), 1–14 (2012). https://doi.org/10.1088/0957-4484/23/7/075102
  21. Q. Xu, X. Guo, L. Xu, Y. Ying, Y. Wu, Y. Wen, H. Yang, Template-free synthesis of SERS-active gold nanopopcorn for rapid detection of chlorpyrifos residues. Sens. Actuators B 241, 1008–1013 (2017). https://doi.org/10.1016/j.snb.2016.11.021
  22. C. Parolo, A. de la Escosura-Muñiz, A. Merkoçi, Enhanced lateral flow immunoassay using gold nanops loaded with enzymes. Biosens. Bioelectron. 40(1), 412–416 (2013). https://doi.org/10.1016/j.bios.2012.06.049
  23. D.H. Choi, S.K. Lee, Y.K. Oh, B.W. Bae, S.D. Lee, S. Kim, Y.B. Shin, M.G. Kim, A dual gold nanop conjugate-based lateral flow assay (LFA) method for the analysis of troponin I. Biosens. Bioelectron. 25(8), 1999–2002 (2010). https://doi.org/10.1016/j.bios.2010.01.019
  24. Z. Ma, S.F. Sui, Naked-eye sensitive detection of immunoglobulin G by enlargement of Au nanops in vitro. Angew. Chem. Int. Ed. Engl. 41(12), 2176–2179 (2002). https://doi.org/10.1002/1521-3773(20020617)41:12<2176:AID-ANIE2176>3.0.CO;2-X
  25. L. Rivas, A. de la Escosura-Muñiz, L. Serrano, L. Altet, O. Francino, A. Sánchez, A. Merkoçi, Triple lines gold nanop-based lateral flow assay for enhanced and simultaneous detection of leishmania DNA and endogenous control. Nano Res. 8(11), 3704–3714 (2015). https://doi.org/10.1007/s12274-015-0870-3
  26. R. Liu, Y. Zhang, S. Zhang, W. Qiu, Y. Gao, Silver enhancement of gold nanops for biosensing: from qualitative to quantitative. App. Spectr. Rev. 49(2), 121–138 (2014). https://doi.org/10.1080/05704928.2013.807817
  27. E.M. Linares, L.T. Kubota, J. Michaelis, S. Thalhammer, Enhancement of the detection limit for lateral flow immunoassays: evaluation and comparison of bioconjugates. J. Immunol. Met. 375(1–2), 264–270 (2012). https://doi.org/10.1016/j.jim.2011.11.003
  28. L. Anfossi, C. Giovannoli, G. Giraudi, F. Biagioli, C. Passini, C. Baggiani, A lateral flow immunoassay for the rapid detection of ochratoxin a in wine and grape must. J. Agric. Food Chem. 60(46), 11491–11497 (2012). https://doi.org/10.1021/jf3031666
  29. G. Frens, Controlled nucleation for the regulation of the p size in monodisperse gold suspensions. Nat. Phys. Sci. 241, 20–22 (1973). https://doi.org/10.1038/physci241020a0
  30. D. Kumar, I. Mutreja, P. Sykes, Seed mediated synthesis of highly monodispersed gold nanops in the presence of hydroquinone. Nanotechnology 27, 355601 (2006). https://doi.org/10.1088/0957-4484/27/35/355601
  31. S.D. Perrault, C.W. Chan, Synthesis and surface modification of highly monodispersed, spherical gold nanops of 50–200 nm. J. Am. Chem. Soc. 131(47), 17042–17043 (2009). https://doi.org/10.1021/ja907069u
  32. C. Kim, H.-M. Song, X. Cai, J. Yao, A. Wei, L.V. Wang, In vivo photoacoustic mapping of lymphatic systems with plasmon-resonant nanostars. J. Mater. Chem. 21(9), 2841–2844 (2011). https://doi.org/10.1039/c0jm04194g
  33. B. Van de Broek, N. Devoogdt, A. D’Hollander, H.-L. Gijs, K. Jans, L. Lagae, S. Muyldermans, G. Maes, G. Borghs, Specific cell targeting with nanobody conjugated branched gold nanops for photothermal therapy. ACS Nano 5(6), 4319–4328 (2011). https://doi.org/10.1021/nn1023363
  34. S.K. Dondapati, T.K. Sau, C. Hrelescu, T.A. Klar, F.D. Stefani, J. Feldmann, Label-free biosensing based on single gold nanostars as plasmonic transducers. ACS Nano 4(11), 6318–6322 (2010). https://doi.org/10.1021/nn100760f
  35. M.O. Rodriguez, L.B. Covian, A.C. Garcia, M.C. Blanco-Lopez, Silver and gold enhancement methods for lateral flow immunoassays. Talanta 148, 272–278 (2015). https://doi.org/10.1016/j.talanta.2015.10.068
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