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Vol. 6 No. 2 (2014)

Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

https://doi.org/10.1007/BF03353778
Guanghui Shen
School of Agricultural and Food Engineering, Shandong University of Technology, Zibo, Shandong 255049, China
Yemin Guo
School of Agricultural and Food Engineering, Shandong University of Technology, Zibo, Shandong 255049, China
Xia Sun
School of Agricultural and Food Engineering, Shandong University of Technology, Zibo, Shandong 255049, China
Xiangyou Wang
School of Agricultural and Food Engineering, Shandong University of Technology, Zibo, Shandong 255049, China

Corresponding Author: Xia Sun

sunxia2151@sina.com

Nano-Micro Letters, Vol. 6 No. 2 (2014), Article Number: 143-152

Abstract

In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline (TET) with prussian blue (PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde (PB-CS-GA) system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor. Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles (AuNPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor. The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry (C-V) and scanning electron microscope (SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry (DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 10−9 ∼ 10−5 M and 10−5 ∼ 10−2 M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×10−10 M (RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

Keywords

Aptasensor Tetracycline Colloidal gold nanoparticle Chitosan
Shen, Guanghui, Yemin Guo, Xia Sun, and Xiangyou Wang. 2014. “Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline”. Nano-Micro Letters 6 (2):143-52. https://doi.org/10.1007/BF03353778.
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