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Vol. 5 No. 3 (2013)

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

https://doi.org/10.1007/BF03353750
Xia Sun
School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P. R. China
Lu Qiao
School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P. R. China
Xiangyou Wang
School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P. R. China

Corresponding Author: Xiangyou Wang

wxy@sdut.edu.cn

Nano-Micro Letters, Vol. 5 No. 3 (2013), Article Number: 191-201

Abstract

A novel multilayer film based on Au nanoparticles (AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite (PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for detecting chlorpyrifos. PANI-coated MWCNTs were prepared by in situ chemical polymerization and carboxylated MWCNTs played an important role in obtaining the thin and uniform coating of PANI resulting in the improved immunosensor response. AuNPs were used as a linker to immobilize chlorpyrifos antibody. The performance of the immunosensor was characterized by means of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM), respectively. All variables involved in the preparation process and analytical performance of the immunosensor were optimized. Under optimal conditions (antibody concentration: 5 μg/ml, working buffer pH: 6.5, incubation time: 40 min, incubation temperature: 25°C), the immunosensor exhibited a wide linear range from 0.1 to 40×10−6 mg/ml and from 40×10−6 mg/ml to 500×10−6 mg/ml, and with a detection limit of 0.06×10−6 mg/ml, which provided a valuable tool for the chlorpyrifos detection in real samples.

Keywords

Immunosensor AuNPs PANI/MWCNTs/CS nanocomposite Chlorpyrifos
Sun, Xia, Lu Qiao, and Xiangyou Wang. 2013. “A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface”. Nano-Micro Letters 5 (3):191-201. https://doi.org/10.1007/BF03353750.
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