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Vol. 7 No. 2 (2015)

Elucidating the Uptake and Distribution of Nanoparticles in Solid Tumors via a Multilayered Cell Culture Model

https://doi.org/10.1007/s40820-014-0025-1
Darren Yohan
Department of Physics, Ryerson University, 350 Victoria Street, Toronto, ON, Canada
Charmainne Cruje
Department of Physics, Ryerson University, 350 Victoria Street, Toronto, ON, Canada
Xiaofeng Lu
Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Toronto, ON, Canada
Devika Chithrani
Department of Physics, Ryerson University, 350 Victoria Street, Toronto, ON, Canada

Corresponding Author: Devika Chithrani

devika.chithrani@ryerson.ca

Nano-Micro Letters, Vol. 7 No. 2 (2015), Article Number: 127-137

Abstract

Multicellular layers (MCLs) have previously been used to determine the pharmacokinetics of a variety of different cancer drugs including paclitaxel, doxorubicin, methotrexate, and 5-fluorouracil across a number of cell lines. It is not known how nanoparticles (NPs) navigate through the tumor microenvironment once they leave the tumor blood vessel. In this study, we used the MCL model to study the uptake and penetration dynamics of NPs. Gold nanoparticles (GNPs) were used as a model system to map the NP distribution within tissue-like structures. Our results show that NP uptake and transport are dependent on the tumor cell type. MDA-MB-231 tissue showed deeper penetration of GNPs as compared to MCF-7 one. Intracellular and extracellular distributions of NPs were mapped using CytoViva imaging. The ability of MCLs to mimic tumor tissue characteristics makes them a useful tool in assessing the efficacy of particle distribution in solid tumors.

Keywords

Gold nanoparticles Tumor Multicellular cell layers Tissue
Yohan, Darren, Charmainne Cruje, Xiaofeng Lu, and Devika Chithrani. 2014. “Elucidating the Uptake and Distribution of Nanoparticles in Solid Tumors via a Multilayered Cell Culture Model”. Nano-Micro Letters 7 (2):127-37. https://doi.org/10.1007/s40820-014-0025-1.
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