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Vol. 13 (2021)

Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells

https://doi.org/10.1007/s40820-021-00661-z
Zhaoyuan Lyu
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Shichao Ding
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Maoyu Wang
School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USA
Xiaoqing Pan
Irvine Materials Research Institute (IMRI), University of California, Irvine, CA 92697, USA
Zhenxing Feng
School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USA
Hangyu Tian
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Chengzhou Zhu
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Dan Du
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Yuehe Lin
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA

Corresponding Author: Yuehe Lin

yuehe.lin@wsu.edu

Nano-Micro Letters, Vol. 13 (2021), Article Number: 146

Abstract

Fe-based single-atomic site catalysts (SASCs), with the natural metalloproteases-like active site structure, have attracted widespread attention in biocatalysis and biosensing. Precisely, controlling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs’ performance. In this work, we use a facile ion-imprinting method (IIM) to synthesize isolated Fe-N-C single-atomic site catalysts (IIM-Fe-SASC). With this method, the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites. The IIM-Fe-SASC shows better peroxidase-like activities than that of non-imprinted references. Due to its excellent properties, IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide (H2O2). Using IIM-Fe-SASC as the nanoprobe, in situ detection of H2O2 generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and specificity. This work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular H2O2 detection.


Highlights:


1 A facile ion-imprinting method (IIM) is used to synthesize the isolated Fe-N-C single-atomic site catalyst (IIM-Fe-SASC), which mimics the natural enzyme-like active site and shows excellent peroxidase-like activity.
2 The ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites.
3 The IIM-Fe-SASC has been successfully used as the nanoprobe for in situ H2O2 detection generated from MDA-MB-231 cells.

Keywords

Single-atomic site catalysts Nanoprobe Peroxidase-like activities Biosensing Living cell
Lyu, Zhaoyuan, Shichao Ding, Maoyu Wang, Xiaoqing Pan, Zhenxing Feng, Hangyu Tian, Chengzhou Zhu, Dan Du, and Yuehe Lin. 2021. “Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells”. Nano-Micro Letters 13 (June):146. https://doi.org/10.1007/s40820-021-00661-z.
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