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Vol. 13 (2021)

Functional Surfactants for Molecular Fishing, Capsule Creation, and Single-Cell Gene Expression

https://doi.org/10.1007/s40820-021-00663-x
Mohammad Suman Chowdhury
Institut Für Chemie Und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany
Xingcai Zhang
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
Leila Amini
Berlin Institute of Health – Center for Regenerative Therapies, Charité Universitätsmedizin Berlin – CVK, Föhrer Str. 15, 13353 Berlin, Germany
Pradip Dey
Institut Für Chemie Und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany
Abhishek Kumar Singh
Institut Für Chemie Und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany
Abbas Faghani
Institut Für Chemie Und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany
Michael Schmueck‑Henneresse
Berlin Institute of Health – Center for Regenerative Therapies, Charité Universitätsmedizin Berlin – CVK, Föhrer Str. 15, 13353 Berlin, Germany
Rainer Haag
Institut Für Chemie Und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany

Corresponding Author: Rainer Haag

haag@chemie.fu-berlin.de

Nano-Micro Letters, Vol. 13 (2021), Article Number: 147

Abstract

Creating a single surfactant that is open to manipulation, while maintaining its surface activity, robustness, and compatibility, to expand the landscape of surfactant-dependent assays is extremely challenging. We report an oxidation-responsive precursor with thioethers and multiple 1,2-diols for creating a variety of functional surfactants from one parent surfactant. Using these multifunctional surfactants, we stabilize microfluidics-generated aqueous droplets. The droplets encapsulate different components and immerse in a bioinert oil with distinct interfaces where an azide-bearing surfactant allow fishing of biomolecules from the droplets, aldehyde-bearing surfactant allow fabrication of microcapsules, and hydroxyl-bearing surfactants, with/without oxidized thioethers, allow monitoring of single-cell gene expression. Creating multifunctional surfactants poses opportunities for broad applications, including adsorption, bioanalytics, catalysis, formulations, coatings, and programmable subset of emulsions.


Highlights:


1 We have demonstrated how to create a parent surfactant that is open to manipulation, while maintaining its surface activity, robustness, and compatibility, to expand the landscape of surfactant-dependent microfluidic-droplet-based assays.
2 To create a library of functional surfactants from the parent surfactant, an oxidation-responsive small building block with thioethers and multiple 1,2-diols was used as a head group, while a non-polar perfluoropolyether chain was used as a tail.

Keywords

Dendronized fluorosurfactants Droplet microfluidics Microcapsules Oxidation-responsive fluorosurfactants Cell encapsulation
Chowdhury, Mohammad Suman, Xingcai Zhang, Leila Amini, Pradip Dey, Abhishek Kumar Singh, Abbas Faghani, Michael Schmueck‑Henneresse, and Rainer Haag. 2021. “Functional Surfactants for Molecular Fishing, Capsule Creation, and Single-Cell Gene Expression”. Nano-Micro Letters 13 (June):147. https://doi.org/10.1007/s40820-021-00663-x.
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