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Vol. 13 (2021)

Human ACE2-Functionalized Gold “Virus-Trap” Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection

https://doi.org/10.1007/s40820-021-00620-8
Yong Yang
State Key Laboratory of High‑Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People’s Republic of China
Yusi Peng
State Key Laboratory of High‑Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People’s Republic of China
Chenglong Lin
State Key Laboratory of High‑Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People’s Republic of China
Li Long
School of Physics and Optoelectronics, South China University of Technology, Guangzhou 510640, People’s Republic of China
Jingying Hu
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200032, People’s Republic of China
Jun He
Anhui Provincial Center for Disease Control and Prevention, Hefei 12560, Anhui, People’s Republic of China
Hui Zeng
Shanghai Yangpu Hospital of Traditional Chinese Medicine, Shanghai 200090, People’s Republic of China
Zhengren Huang
State Key Laboratory of High‑Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People’s Republic of China
Zhi‑Yuan Li
School of Physics and Optoelectronics, South China University of Technology, Guangzhou 510640, People’s Republic of China
Masaki Tanemura
Department of Frontier Materials, Nagoya Institute of Technology, Nagoya 466‑8555, Japan
Jianlin Shi
State Key Laboratory of High‑Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People’s Republic of China
John R. Lombardi
Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA
Xiaoying Luo
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200032, People’s Republic of China

Corresponding Author: Xiaoying Luo

luoxy@shsci.org

Nano-Micro Letters, Vol. 13 (2021), Article Number: 109

Abstract

The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold “virus traps” nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 106-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as “virus-traps” composed of oblique gold nanoneedles, and 109-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL−1 for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.


Highlights:


1 Extremely sensitive and prompt COVID-19 SERS biosensor to detect SARS-CoV-2 virus in the contaminated water at single-virus level.
2 SERS sensor features a low detection limit of 80 copies mL−1 for the simulated contaminated water by SARS-CoV-2 virus as short as 5 min.
3 ACE2-modified SERS sensor with machine-learning and identification standard enable rapid detection of novel yet-unknown coronaviruses.

Keywords

SERS SARS-CoV-2 Human ACE2 “Virus-trap” nanostructure Single-virus detection
Yang, Yong, Yusi Peng, Chenglong Lin, Li Long, Jingying Hu, Jun He, Hui Zeng, Zhengren Huang, Zhi‑Yuan Li, Masaki Tanemura, Jianlin Shi, John R. Lombardi, and Xiaoying Luo. 2021. “Human ACE2-Functionalized Gold ‘Virus-Trap’ Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection”. Nano-Micro Letters 13 (April):109. https://doi.org/10.1007/s40820-021-00620-8.
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